Communications between distant sites on supercoiled DNA from non-exponential kinetics for DNA synapsis by resolvase.

@article{Oram1997CommunicationsBD,
  title={Communications between distant sites on supercoiled DNA from non-exponential kinetics for DNA synapsis by resolvase.},
  author={M. Oram and John Frederick Daniel Marko and Stephen E. Halford},
  journal={Journal of molecular biology},
  year={1997},
  volume={270 3},
  pages={396-412}
}
To determine how distant sites on supercoiled DNA communicate with each other, the mechanism of site-specific recombination by resolvase was analysed by using a rapid-reaction quench-flow device to study the kinetics of individual steps in the reaction pathway. Three sets of measurements revealed the rates for: (1) the initial binding of the protein to its target sites on the DNA; (2) the synapsis of the two DNA-protein complexes; (3) the overall process of recombination. The binding of the… CONTINUE READING

From This Paper

Topics from this paper.
11 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations

Similar Papers

Loading similar papers…