Commonly Used Reference Genes are Actively Regulated in In vitro Stimulated Lymphocytes

  title={Commonly Used Reference Genes are Actively Regulated in In vitro Stimulated Lymphocytes},
  author={R. R{\o}ge and J. Thorsen and C. T{\o}rring and A. {\O}zbay and B. K. M{\o}ller and J. Carstens},
  journal={Scandinavian Journal of Immunology},
  • R. Røge, J. Thorsen, +3 authors J. Carstens
  • Published 2007
  • Biology, Medicine
  • Scandinavian Journal of Immunology
  • Quantitative polymerase chain reaction (Q‐PCR) studies of urine sediments have demonstrated an increased expression of cytotoxin genes during episodes of acute rejection of renal allografts. To compensate for differences in initial sample size and cDNA preparation, standard Q‐PCR experiments involve normalization to a reference gene. Although stable expression of reference genes is a prerequisite for any Q‐PCR analysis, commonly used reference genes have demonstrated a varying expression across… CONTINUE READING
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