Commercially available antibodies against human and murine histamine H4-receptor lack specificity

@article{Beermann2011CommerciallyAA,
  title={Commercially available antibodies against human and murine histamine H4-receptor lack specificity},
  author={Silke Beermann and Roland Seifert and Detlef Neumann},
  journal={Naunyn-Schmiedeberg's Archives of Pharmacology},
  year={2011},
  volume={385},
  pages={125-135}
}
Antibodies are important tools to detect expression and localization of proteins within the living cell. However, for a series of commercially available antibodies which are supposed to recognize G-protein-coupled receptors (GPCR), lack of specificity has been described. In recent publications, antisera against the histamine H4-receptor (H4R), which is a member of the GPCR family, have been used to demonstrate receptor expression. However, a comprehensive characterization of these antisera has… CONTINUE READING

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