Commercial-scale sperm cryopreservation of diploid and tetraploid Pacific oysters, Crassostrea gigas.

@article{Dong2005CommercialscaleSC,
  title={Commercial-scale sperm cryopreservation of diploid and tetraploid Pacific oysters, Crassostrea gigas.},
  author={Qiaoxiang Dong and Benoit Eudeline and Changjiang Huang and Standish K. Allen and T. R. Tiersch},
  journal={Cryobiology},
  year={2005},
  volume={50 1},
  pages={1-16}
}
Cryopreservation of sperm from tetraploid organisms (the possession of four chromosome sets) is essentially unexplored. This is the first cryopreservation study to address sperm from tetraploid Pacific oysters, Crassostrea gigas, and addresses the commercial production of triploid oysters (three chromosome sets). Initial motility, refrigerated storage of undiluted sperm, osmolality of extender solutions, sperm concentrations, equilibration time, and cryoprotectants of propylene glycol and… CONTINUE READING
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