Combining real-time polymerase chain reaction using SYBR Green I detection and sequencing to identify vertebrate bloodmeals in fleas.

@article{Graham2012CombiningRP,
  title={Combining real-time polymerase chain reaction using SYBR Green I detection and sequencing to identify vertebrate bloodmeals in fleas.},
  author={Christine B Graham and William C. Black and Karen A. Boegler and John A. Montenieri and Jennifer L. Holmes and Kenneth L. Gage and Rebecca J. Eisen},
  journal={Journal of medical entomology},
  year={2012},
  volume={49 6},
  pages={1442-52}
}
Programs that aim to control vector-borne zoonotic diseases require information on zoonotic hosts and on the feeding behavior of bridging vectors that are capable of transmitting pathogens from those hosts to humans. Here we describe an assay developed to identify bloodmeals in field-collected cat fleas (Ctenocephalides felis Bouché) to assess this species' potential role as a Yersinia pestis bridging vector in a plague-endemic region of Uganda. Our assay uses a single primer set and SYBR Green… CONTINUE READING