Combining protein complementation assays with resonance energy transfer to detect multipartner protein complexes in living cells.

@article{Rebois2008CombiningPC,
  title={Combining protein complementation assays with resonance energy transfer to detect multipartner protein complexes in living cells.},
  author={Raymond V. Rebois and M{\'e}lanie Robitaille and Darlaine P{\'e}trin and Peter Zylbergold and Phan Trieu and Terence E. H{\'e}bert},
  journal={Methods},
  year={2008},
  volume={45 3},
  pages={214-8}
}
A variety of fluorescent proteins with different spectral properties have been created by mutating green fluorescent protein. When these proteins are split in two, neither fragment is fluorescent per se, nor can a fluorescent protein be reconstituted by co-expressing the complementary N- and C-terminal fragments. However, when these fragments are genetically fused to proteins that associate with each other in cellulo, the N- and C-terminal fragments of the fluorescent protein are brought… CONTINUE READING
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