Combining multispectral polarized light imaging and confocal microscopy for localization of nonmelanoma skin cancer.

@article{Yaroslavsky2005CombiningMP,
  title={Combining multispectral polarized light imaging and confocal microscopy for localization of nonmelanoma skin cancer.},
  author={Anna N. Yaroslavsky and Jos{\'e} Micael Delgado Barbosa and Victor A. Neel and Charles A. DiMarzio and R Rox Anderson},
  journal={Journal of biomedical optics},
  year={2005},
  volume={10 1},
  pages={
          14011
        }
}
Multispectral polarized light imaging (MSPLI) enables rapid inspection of a superficial tissue layer over large surfaces, but does not provide information on cellular microstructure. Confocal microscopy (CM) allows imaging within turbid media with resolution comparable to that of histology, but suffers from a small field of view. In practice, pathologists use microscopes at low and high power to view tumor margins and cell features, respectively. Therefore, we study the combination of CM and… 
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References

SHOWING 1-10 OF 19 REFERENCES
Confocal examination of nonmelanoma cancers in thick skin excisions to potentially guide mohs micrographic surgery without frozen histopathology.
TLDR
Real-time confocal reflectance microscopy offers an imaging method potentially to avoid frozen histopathology and prepare noninvasive (optical) sections within 5 min and potentially save several hours per day in the operating room.
Demarcation of nonmelanoma skin cancer margins in thick excisions using multispectral polarized light imaging.
TLDR
A novel multispectral dye-enhanced polarized light imaging technique that enables rapid imaging of large tumor fields and demonstrates that staining significantly enhances contrast of the tumor in the image and enables reliable delineation of cancer.
In vivo confocal scanning laser microscopy of human skin: melanin provides strong contrast.
TLDR
In vivo confocal microscopy potentially offers dermatologists a diagnostic tool that is instant and entirely non-invasive compared to conventional histopathology.
Laser scanning confocal microscopy of cervical tissue before and after application of acetic acid.
TLDR
High-contrast reflected light images of cervical tissue are attainable in near real time and acetic acid significantly increases light scattering from cell nuclei, which may partially explain why acetowhitening occurs.
Video-rate confocal scanning laser microscope for imaging human tissues in vivo.
TLDR
A video-rate confocal scanning laser microscope for reflectance imaging of human skin and oral mucosa in vivo with comparable resolution and contrast to that of conventional microscopy of excised biopsies (histology).
Intraoperative assessment of microsurgery with three-dimensional optical coherence tomography.
TLDR
Three-dimensional, micrometer-scale, diagnostic imaging capabilities of OCT permit rapid feedback for assessment of microsurgical procedures and can be readily integrated with surgical microscopes and has potential for intraoperative monitoring to improve patient outcome.
In vivo imaging of human teeth and skin using real-time confocal microscopy
TLDR
In vivo applications of the tandem scanning microscope (TSM) can be applied in the in vivo microscopic evaluation of living tissue in the study of human teeth and skin.
Intramitochondrial dyes allow selective in vitro photolysis of carcinoma cells.
TLDR
The most effective, N,N'-bis(2-ethyl-1,3-dioxolane)kryptocyanine (EDKC), caused marked, light-dependent killing of human bladder, squamous, and colon carcinoma cell lines after 30-min incubations at 1-0.01 microM but was minimally toxic to human keratinocytes and to normal monkey kidney epithelial cells (CV-1).
Detection of preinvasive cancer cells
TLDR
A new optical-probe technique based on light-scattering spectroscopy that is able to detect precancerous and early cancerous changes in cell-rich epithelia is presented.
Imaging superficial tissues with polarized light
TLDR
This study presents a study of the transition of linearly polarized light into randomly polarized light during light propagation through tissues, and some example images are presented.
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