Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control.

@article{Leonard2010CombiningMA,
  title={Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control.},
  author={Effendi Leonard and Parayil Kumaran Ajikumar and Kelly Thayer and Wenhai Xiao and Jeffrey D Mo and Bruce Tidor and Gregory Stephanopoulos and Kristala Jones Prather},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2010},
  volume={107 31},
  pages={13654-9}
}
A common strategy of metabolic engineering is to increase the endogenous supply of precursor metabolites to improve pathway productivity. The ability to further enhance heterologous production of a desired compound may be limited by the inherent capacity of the imported pathway to accommodate high precursor supply. Here, we present engineered diterpenoid biosynthesis as a case where insufficient downstream pathway capacity limits high-level levopimaradiene production in Escherichia coli. To… CONTINUE READING
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Stabilizedgeneduplicationenables long - term selection - free heterologous pathway expression

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