Combining laser capture microdissection with quantitative real-time PCR: effects of tissue manipulation on RNA quality and gene expression.

@article{Kerman2006CombiningLC,
  title={Combining laser capture microdissection with quantitative real-time PCR: effects of tissue manipulation on RNA quality and gene expression.},
  author={Ilan A. Kerman and Bradley J. Buck and Simon J. Evans and Huda Akil and Stanley J. Watson},
  journal={Journal of neuroscience methods},
  year={2006},
  volume={153 1},
  pages={71-85}
}
Laser capture microdissection (LCM) is increasingly being used in quantitative gene expression studies of the nervous system. The current study aimed at determining the impact of various tissue manipulations on the integrity of extracted RNA in LCM studies. Our data indicate that various tissue preparation strategies prior to microdissection may decrease RNA quality by as much as 25%, thus affecting expression profiles of some genes. To circumvent this problem, we developed a strategy for… CONTINUE READING

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