Combining isoelectric point-based fractionation, liquid chromatography and mass spectrometry to improve peptide detection and protein identification.

@article{Cologna2010CombiningIP,
  title={Combining isoelectric point-based fractionation, liquid chromatography and mass spectrometry to improve peptide detection and protein identification.},
  author={Stephanie M. Cologna and William K. Russell and Peniel J Lim and Gyula Vigh and David H Russell},
  journal={Journal of the American Society for Mass Spectrometry},
  year={2010},
  volume={21 9},
  pages={1612-9}
}
The off-line coupling of an isoelectric trapping device termed membrane separated wells for isoelectric focusing and trapping (MSWIFT) to mass spectrometry-based proteomic studies is described. The MSWIFT is a high capacity, high-throughput, mass spectrometry-compatible isoelectric trapping device that provides isoelectric point (pI)-based separations of complex mixtures of peptides. In MSWIFT, separation and analyte trapping are achieved by migrating the peptide ions through membranes having… CONTINUE READING

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