Combining high-throughput sequencing with fruit body surveys reveals contrasting life-history strategies in fungi

@article{Ovaskainen2013CombiningHS,
  title={Combining high-throughput sequencing with fruit body surveys reveals contrasting life-history strategies in fungi},
  author={Otso Ovaskainen and Dmitry Schigel and Heini Ali-Kovero and Petri Auvinen and Lars Paulin and Bj{\"o}rn Nord{\'e}n and Jenni Nord{\'e}n},
  journal={The ISME Journal},
  year={2013},
  volume={7},
  pages={1696-1709}
}
Before the recent revolution in molecular biology, field studies on fungal communities were mostly confined to fruit bodies, whereas mycelial interactions were studied in the laboratory. Here we combine high-throughput sequencing with a fruit body inventory to study simultaneously mycelial and fruit body occurrences in a community of fungi inhabiting dead wood of Norway spruce. We studied mycelial occurrence by extracting DNA from wood samples followed by 454-sequencing of the ITS1 and ITS2… CONTINUE READING
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