Combining different design strategies for rational affinity maturation of the MICA-NKG2D interface.

@article{Henager2012CombiningDD,
  title={Combining different design strategies for rational affinity maturation of the MICA-NKG2D interface.},
  author={Samuel H. Henager and Melissa A Hale and Nicholas J Maurice and Erin C Dunnington and Carter J Swanson and Megan J. Peterson and Joseph J Ban and David J Culpepper and Luke D Davies and Lisa K Sanders and Benjamin J McFarland},
  journal={Protein science : a publication of the Protein Society},
  year={2012},
  volume={21 9},
  pages={1396-402}
}
We redesigned residues on the surface of MICA, a protein that binds the homodimeric immunoreceptor NKG2D, to increase binding affinity with a series of rational, incremental changes. A fixed-backbone RosettaDesign protocol scored a set of initial mutations, which we tested by surface plasmon resonance for thermodynamics and kinetics of NKG2D binding, both… CONTINUE READING