Combining confocal and BSE SEM imaging for bone block surfaces.

  title={Combining confocal and BSE SEM imaging for bone block surfaces.},
  author={Alan Boyde and Luděk Lovicar and Jan Zamecnik},
  journal={European cells & materials},
The present report presents a method for the correlation of qualitative and quantitative BSE SEM imaging with confocal scanning light microscopy (CSLM) imaging modes applied to bone samples embedded in PMMA. The SEM has a proper digital scan generator: we leave the BSE image unchanged, and match the CSLM image to it, because the CSLM scan mechanism is not digital, though the signal is digitised. Our overlapping program uses a linear transformation matrix which projects one system to the other… CONTINUE READING