Combining Voltage and Calcium Imaging from Neuronal Dendrites

  title={Combining Voltage and Calcium Imaging from Neuronal Dendrites},
  author={Marco Canepari and Kaspar Vogt and Dejan Zecevic},
  journal={Cellular and Molecular Neurobiology},
The ability to monitor membrane potential (V m) and calcium (Ca2+) transients at multiple locations on the same neuron can facilitate further progress in our understanding of neuronal function. Here we describe a method to combine V m and Ca2+ imaging using styryl voltage sensitive dyes and Fura type UV-excitable Ca2+ indicators. In all cases V m optical signals are linear with membrane potential changes, but the calibration of optical signals on an absolute scale is presently possible only in… CONTINUE READING
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