Combining RNA-DNA swapping and quantitative polymerase chain reaction for the detection of influenza A nucleoprotein.

@article{Morin2012CombiningRS,
  title={Combining RNA-DNA swapping and quantitative polymerase chain reaction for the detection of influenza A nucleoprotein.},
  author={Isabelle Morin and Patrick M. Schaeffer},
  journal={Analytical biochemistry},
  year={2012},
  volume={420 2},
  pages={121-6}
}
The detection of proteinaceous antigens generally relies on traditional immunoassays and, more recently, on immuno-PCR (polymerase chain reaction) assays and their derivatives, which do not take advantage of the intrinsic function or binding property of a protein. The RNA-binding nucleoprotein has been shown to be an excellent target for the development of various influenza A diagnostics due to its high antigenicity and the presence of large numbers in the virus. It binds nonspecifically to the… CONTINUE READING
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