Combining Mutations and Electrophysiology to Map Anesthetic Sites on Ligand-Gated Ion Channels.

@article{Forman2018CombiningMA,
  title={Combining Mutations and Electrophysiology to Map Anesthetic Sites on Ligand-Gated Ion Channels.},
  author={Stuart A. Forman},
  journal={Methods in enzymology},
  year={2018},
  volume={602},
  pages={
          369-389
        }
}
  • S. Forman
  • Published 2018
  • Chemistry, Medicine
  • Methods in enzymology
General anesthetics are known to act in part by binding to and altering the function of pentameric ligand-gated ion channels such as nicotinic acetylcholine and γ-aminobutyric acid type A receptors. Combining heterologous expression of the subunits that assemble to form these ion channels, mutagenesis techniques and voltage-clamp electrophysiology have enabled a variety of "structure-function" approaches to questions of where anesthetic binds to these ion channels and how they enhance or… Expand
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