Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

@inproceedings{Duncan2011CombiningLD,
  title={Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology},
  author={Jeremy S Duncan and Jennifer Kersigo and Brian D. Gray and Bernd Fritzsch},
  booktitle={Journal of visualized experiments : JoVE},
  year={2011}
}
Going beyond single gene function to cut deeper into gene regulatory networks requires multiple mutations combined in a single animal. Such analysis of two or more genes needs to be complemented with in situ hybridization of other genes, or immunohistochemistry of their proteins, both in whole mounted developing organs or sections for detailed resolution of the cellular and tissue expression alterations. Combining multiple gene alterations requires the use of cre or flipase to conditionally… CONTINUE READING

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