Combining FISH with localisation microscopy: Super-resolution imaging of nuclear genome nanostructures

@article{Weiland2010CombiningFW,
  title={Combining FISH with localisation microscopy: Super-resolution imaging of nuclear genome nanostructures},
  author={Yanina Weiland and Paul Lemmer and Christoph Cremer},
  journal={Chromosome Research},
  year={2010},
  volume={19},
  pages={5-23}
}
The optical resolution of conventional far field fluorescence light microscopy is restricted to about 200 nm laterally and 600 nm axially and has been thought for many decades to be an insurmountable barrier for the quantitative spatial analysis of cellular and hence also nuclear constituents. Novel approaches in light microscopy have now overcome this… CONTINUE READING