Combined video fluorescence and 3D electron microscopy.

  title={Combined video fluorescence and 3D electron microscopy.},
  author={Alexander A. Mironov and Roman S. Polishchuk and Galina V. Beznoussenko},
  journal={Methods in cell biology},
Three-dimensional structure of cells and organelles examined with the power of resolution of electron microscopy (EM) including EM tomography represents the average view of cell processes. More precise and detailed analysis is limited by the significant variations in the structure of cells in temporal dynamics of cellular events. Therefore EM cannot identify rare and fast events that could be extremely important for understanding molecular mechanisms underlying these cellular processes… CONTINUE READING