Combined use of growth rate correlated and growth rate independent promoters for recombinant glucoamylase production in Fusarium venenatum.

@article{Gordon2001CombinedUO,
  title={Combined use of growth rate correlated and growth rate independent promoters for recombinant glucoamylase production in Fusarium venenatum.},
  author={C L Gordon and S. Thomas and Alison M. Griffen and G. D. Robson and Anthony P. J. Trinci and Marilyn G Wiebe},
  journal={FEMS microbiology letters},
  year={2001},
  volume={194 2},
  pages={
          229-34
        }
}
Fusarium venenatum JeRS 325, a strain which produces recombinant glucoamylase under control of a growth rate independent promoter was transformed with a plasmid carrying the Aspergillus niger glucoamylase gene under control of its own growth rate correlated promoter. Some disruption of the original recombinant genes occurred and at pH 5.8 the double transformant did not produce as much glucoamylase as JeRS 325 in batch culture. However, the double transformant still produced as much… 
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TLDR
The results suggested that a chemostat operated at a slow dilution rate would be the most productive culture system for enzyme production under this trypsin‐like promoter.
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