Combined subtractive cDNA cloning and array CGH: an efficient approach for identification of overexpressed genes in DNA amplicons

@article{Preter2003CombinedSC,
  title={Combined subtractive cDNA cloning and array CGH: an efficient approach for identification of overexpressed genes in DNA amplicons},
  author={Katleen De Preter and Filip Pattyn and Geert Berx and Kristin Strumane and Bj{\"o}rn Menten and Frans van Roy and Anne De Paepe and Frank Speleman and Jo Vandesompele},
  journal={BMC Genomics},
  year={2003},
  volume={5},
  pages={11 - 11}
}
Activation of proto-oncogenes by DNA amplification is an important mechanism in the development and maintenance of cancer cells. Until recently, identification of the targeted genes relied on labour intensive and time consuming positional cloning methods. In this study, we outline a straightforward and efficient strategy for fast and comprehensive cloning of amplified and overexpressed genes. As a proof of principle, we analyzed neuroblastoma cell line IMR-32, with at least two amplification… CONTINUE READING
12 Citations
35 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 12 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 35 references

Similar Papers

Loading similar papers…