Combined ribosomal DNA and morphological analysis of individual gyrodactylid monogeneans.

@article{Harris1999CombinedRD,
  title={Combined ribosomal DNA and morphological analysis of individual gyrodactylid monogeneans.},
  author={Philip David Harris and Joanne Cable and Richard C. Tinsley and Colin Michael Lazarus},
  journal={The Journal of parasitology},
  year={1999},
  volume={85 2},
  pages={188-91}
}
A method is presented for the isolation and analysis of hamuli, marginal hooks, and bars from individual gyrodactylid monogeneans using scanning electron microscopy (SEM), while simultaneously processing parasites for rDNA analysis using the polymerase chain reaction (PCR). The haptors of ethanol-fixed gyrodactylids were protease digested to liberate hooks for SEM, whereas DNA extracted from the bodies was used for PCR. The method resulted in hooks and hamuli being prepared from more than 90… CONTINUE READING

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