Combined organ of Corti/modiolus technique for preparing mammalian cochleas for quantitative microscopy

@article{Bohne1993CombinedOO,
  title={Combined organ of Corti/modiolus technique for preparing mammalian cochleas for quantitative microscopy},
  author={Barbara Ann Bohne and Gary W. Harding},
  journal={Hearing Research},
  year={1993},
  volume={71},
  pages={114-124}
}
An improved cochlear preparation technique is described with the following key features: 1) Preservation of the organ of Corti (OC) and the spiral ganglion cells (SGCs) in the same cochlea for quantitative, high-resolution microscopic evaluation; 2) Dissection of the plastic-embedded cochlea so that the entire OC can be prepared as whole mounts for quantitative study while the modiolus remains in a single block; 3) Decalcification and serial sectioning of the modiolus so that all SGC bodies are… CONTINUE READING
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