Combined nickel-cobalt-cadmium resistance encoded by the ncc locus of Alcaligenes xylosoxidans 31A

@article{Schmidt1994CombinedNR,
  title={Combined nickel-cobalt-cadmium resistance encoded by the ncc locus of Alcaligenes xylosoxidans 31A},
  author={Th. Schmidt and Hans G{\"u}nter Schlegel},
  journal={Journal of Bacteriology},
  year={1994},
  volume={176},
  pages={7045 - 7054}
}
The nickel-cobalt-cadmium resistance genes carried by plasmid pTOM9 of Alcaligenes xylosoxidans 31A are located on a 14.5-kb BamHI fragment. By random Tn5 insertion mutagenesis, the fragment was shown to contain two distinct nickel resistance loci, ncc and nre. The ncc locus causes a high-level combined nickel, cobalt, and cadmium resistance in strain AE104, which is a cured derivative of the metal-resistant bacterium Alcaligenes eutrophus CH34. ncc is not expressed in Escherichia coli. The nre… Expand
Regulation of the cnr Cobalt and Nickel Resistance Determinant of Ralstonia eutropha (Alcaligenes eutrophus) CH34
TLDR
The linked resistance to nickel and cobalt of Ralstonia eutropha-like strain CH34 is encoded by the cnr operon, which is localized on the megaplasmid pMOL28, and transcriptional fusions were constructed between fragments of cnrYXH and the luxCDABE, luciferase reporter genes. Expand
MrdH, a Novel Metal Resistance Determinant of Pseudomonas putida KT2440, Is Flanked by Metal-Inducible Mobile Genetic Elements
TLDR
This study is the first report of a single-component metal efflux transporter, mrdH, showing chimeric domain organization, a broad substrate spectrum, and a location amid metal-inducible mobile genetic elements. Expand
Regulation of the cnr Cobalt and Nickel Resistance Determinant from Ralstonia sp. Strain CH34
TLDR
Half-maximal activation from the cnrCBA operon was determined by using appropriate lacZ gene fusions and was shown to occur at an Ni(2+) concentration of about 50 microM. Expand
Amplified rDNA restriction analysis and further genotypic characterisation of metal-resistant soil bacteria and related facultative hydrogenotrophs.
TLDR
The analysis showed a close phylogenetic clustering of the czc+ strains inside the Ralstonia genus despite of their different origins, which suggests that the tested czC+ strains and some of the ncc+ strains may be considered as belonging to the genus RalSTONia. Expand
The genetic basis of cadmium resistance of Burkholderia cenocepacia.
TLDR
Inoculation of the HM-hyperaccumulating plant Arabidopsis halleri with a RFP-marked derivative of Burkholderia cenocepacia H111 containing the PcadA -gfp fusion demonstrated the applicability of this biosensor for monitoring cadmium at the single cell level in a natural environment. Expand
Nucleotide sequence and expression of the ncr nickel and cobalt resistance in Hafnia alvei 5-5.
TLDR
The structural genes for the nickel and cobalt resistance of the conjugative plasmid pEJH 501 of Hafnia alvei 5-5, contained on a SalI-EcoRI fragment of 4.8 kb, were cloned and sequenced and found to be homologous to the amino acid sequences of Achromobacter xylosoxidans 31A. Expand
Identification of a gene cluster, czr, involved in cadmium and zinc resistance in Pseudomonas aeruginosa.
TLDR
A high sequence identity and organizatory conservation of the czr region of CMG103 was found in PAO1 as well regarding coding sequences as intervening sequences between ORFs, and DNA-DNA hybridization indicated strong conservation of czr in other environmental P. aeruginosa isolates. Expand
Two RND proteins involved in heavy metal efflux in Caulobacter crescentus belong to separate clusters within proteobacteria
TLDR
The czrCBA efflux system is involved mainly in response to cadmium and zinc with a secondary role inresponse to cobalt. Expand
Megaplasmids in Cupriavidus Genus and Metal Resistance
Megaplasmids carrying genetic determinants for the response and the resistance to heavy metals are regularly found in bacteria belonging to the genus Cupriavidus/Ralstonia and isolated fromExpand
Inducible nickel resistance in a river isolate of India phylogenetically ascertained as a novel strain of Acinetobacter junii
TLDR
The isolate BB1A carries out a novel induction phenomenon of nickel resistance and presumably with a nickel resistance genetic system different from that previously characterized in other bacteria. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 46 REFERENCES
Characterization of the inducible nickel and cobalt resistance determinant cnr from pMOL28 of Alcaligenes eutrophus CH34
TLDR
From pMOL28, one of the two heavy metal resistance plasmids of Alcaligenes eutrophus strain CH34, an EcoRI-PstI fragment was cloned into plasmid pVDZ'2 and mutations located upstream of cnrC resulted in various phenotypic changes, which resulted in two- to fivefold-increased nickel resistance in both hosts. Expand
High-Level Nickel Resistance in Alcaligenes xylosoxydans 31A and Alcaligenes eutrophus KTO2
Two new nickel-resistant strains of Alcaligenes species were selected from a large number (about 400) of strains isolated from ecosystems polluted by heavy metals and were studied on theExpand
Cloning of pMOL28-encoded nickel resistance genes and expression of the genes in Alcaligenes eutrophus and Pseudomonas spp
TLDR
DNA sequences responsible for nickel resistance in newly isolated strains showed homology to the cloned pMOL28-encoded nickel and cobalt resistance determinant. Expand
A new type of Alcaligenes eutrophus CH34 zinc resistance generated by mutations affecting regulation of the cnr cobalt-nickel resistance system
TLDR
Uptake studies showed that the zinc resistance in a Znr mutant resulted from reduced accumulation of zinc ions in comparison with that in the plasmid-free strain, suggesting that zinc interferes with the Ni2+ and Co2+ efflux system. Expand
Cloning and expression of plasmid genes encoding resistances to chromate and cobalt in Alcaligenes eutrophus
TLDR
The resistances expressed from the hybrid plasmids after transfer back into A. eutrophus were inducible and conferred the same degree of resistance as the parent plasmid pMOL28, and were expressed in metal-sensitive Alcaligenes strains and related bacteria but not in Escherichia coli. Expand
Cloning of plasmid genes encoding resistance to cadmium, zinc, and cobalt in Alcaligenes eutrophus CH34
TLDR
A 238-kilobase-pair plasmid, pMOL30, confers resistance to cadmium, zinc, and cobalt salts in Alcaligenes eutrophus CH34 and is cloned into the broad-host-range hybrid plasmID pRK290. Expand
Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals
Alcaligenes eutrophus strain CH34, which was isolated as a bacterium resistant to cobalt, zinc, and cadmium ions, shares with A. eutrophus strain H16 the ability to grow lithoautotrophically onExpand
Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus.
Resistance to cobalt, zinc, and cadmium specified by the czc determinant on plasmid pMOL30 in Alcaligenes eutrophus results from a cation efflux system. Five membrane-bound polypeptides that wereExpand
CzcR and CzcD, gene products affecting regulation of resistance to cobalt, zinc, and cadmium (czc system) in Alcaligenes eutrophus
  • D. Nies
  • Biology, Medicine
  • Journal of bacteriology
  • 1992
TLDR
The czcR gene, one of the two control genes responsible for induction of resistance to Co2+, Zn2+, and Cd2+ (czc system) in the Alcaligenes eutrophus plasmid pMOL30, was cloned and characterized and the membrane-bound CzcD protein was essential for inductionof czc. Expand
Plasmid pMOL28-encoded resistance to nickel is due to specific efflux
TLDR
The results suggest that in M243 under normal aerobic conditions two constitutive energy-dependent cation transport systems are functioning concomitantly; a chromosomally determined nickel uptake system and a plasmid-mediated nickel efflux system. Expand
...
1
2
3
4
5
...