Combined molecular and biochemical approach identifies Aspergillus japonicus and Aspergillus aculeatus as two species.

@article{Parenicov2001CombinedMA,
  title={Combined molecular and biochemical approach identifies Aspergillus japonicus and Aspergillus aculeatus as two species.},
  author={Lucie Parenicov{\'a} and Pernille Skouboe and Jens C Frisvad and Robert a. Samson and Lone Rossen and M ten Hoor-Suykerbuyk and J. Arjan G. M. de Visser},
  journal={Applied and environmental microbiology},
  year={2001},
  volume={67 2},
  pages={
          521-7
        }
}
We examined nine Aspergillus japonicus isolates and 10 Aspergillus aculeatus isolates by using molecular and biochemical markers, including DNA sequences of the ITS1-5.8S rRNA gene-ITS2 region, restriction fragment length polymorphisms (RFLP), and secondary-metabolite profiles. The DNA sequence of the internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene could not be used to distinguish between A. japonicus and A. aculeatus but did show that these two taxa are more closely related… CONTINUE READING
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