Combined micromanipulation, culture and immunofluorescent techniques for isolation of the coccal organisms comprising the "corn cob" configuration of human dental plaque.

@article{Mouton1977CombinedMC,
  title={Combined micromanipulation, culture and immunofluorescent techniques for isolation of the coccal organisms comprising the "corn cob" configuration of human dental plaque.},
  author={Christian Mouton and Homer S. Reynolds and Robert J. Genco},
  journal={Journal de biologie buccale},
  year={1977},
  volume={5 4},
  pages={
          321-32
        }
}
The present study describes methods to 1) selectively isolate Corn Cob Configurations (CCC) from dental plaque by micromanipulation; 2) obtain pure cultures of the coccal constituent; 3) determine by immunofluorescent procedure which organisms originated from the CCC. Using a de Fonbrune micromanipulator, CCC specimens were isolated from supragingival plaque samples. The viability of one specimen thus obtained was established by observing growth on a slide culture. One set of CCC specimens was… CONTINUE READING

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