Combined imaging of bacteria and oxygen in biofilms.

  title={Combined imaging of bacteria and oxygen in biofilms.},
  author={Michael K{\"u}hl and Lars Fledelius Rickelt and Roland Thar},
  journal={Applied and environmental microbiology},
  volume={73 19},
Transparent sensors for microscopic O(2) imaging were developed by spin coating an ultrathin (<1- to 2-microm) layer of a luminescent O(2) indicator onto coverslips. The sensors showed (i) an ideal Stern-Volmer quenching behavior of the luminescence lifetime towards O(2) levels, (ii) homogeneous measuring characteristics over the sensor surface, and (iii) a linear decline of luminescence lifetime with increasing temperature. When a batch of such coverslip sensors has been characterized, their… CONTINUE READING