Combined Species Identification, Genotyping, and Drug Resistance Detection of Mycobacterium tuberculosis Cultures by MLPA on a Bead-Based Array

@inproceedings{Bergval2012CombinedSI,
  title={Combined Species Identification, Genotyping, and Drug Resistance Detection of Mycobacterium tuberculosis Cultures by MLPA on a Bead-Based Array},
  author={Indra L. Bergval and Sarah Sengstake and Nadia Brankova and Viktoria Levterova and Edgar Abad{\'i}a and Nino Tadumaze and Nino Bablishvili and M. Ia. Akhalaia and Kiki Tuin and Anja R. J. Schuitema and Stefan Panaiotov and Elizabeta Bachiyska and Todor Kantardjiev and Rina de Zwaan and Anita C. Sch{\"u}rch and Dick van Soolingen and Anja van ‘t Hoog and Frank Cobelens and Rusudan Aspindzelashvili and Christophe Sola and Paul R. Klatser and Richard Anthony},
  booktitle={PloS one},
  year={2012}
}
The population structure of Mycobacterium tuberculosis is typically clonal therefore genotypic lineages can be unequivocally identified by characteristic markers such as mutations or genomic deletions. In addition, drug resistance is mainly mediated by mutations. These issues make multiplexed detection of selected mutations potentially a very powerful tool to characterise Mycobacterium tuberculosis. We used Multiplex Ligation-dependent Probe Amplification (MLPA) to screen for dispersed… CONTINUE READING

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