Combined Proteomic and Metabolomic Profiling of the Arabidopsis thaliana vps29 Mutant Reveals Pleiotropic Functions of the Retromer in Seed Development

  title={Combined Proteomic and Metabolomic Profiling of the Arabidopsis thaliana vps29 Mutant Reveals Pleiotropic Functions of the Retromer in Seed Development},
  author={Thomas C. Durand and Gwendal Cueff and B{\'e}atrice Godin and Beno{\^i}t Valot and Gilles Cl{\'e}ment and Thierry Gaude and Lo{\"i}c Rajjou},
  journal={International Journal of Molecular Sciences},
The retromer is a multiprotein complex conserved from yeast to humans, which is involved in intracellular protein trafficking and protein recycling. Selection of cargo proteins transported by the retromer depends on the core retromer subunit composed of the three vacuolar protein sorting (VPS) proteins, namely VPS26, VPS29, and VPS35. To gain a better knowledge of the importance of the plant retromer in protein sorting, we carried out a comparative proteomic and metabolomic analysis of… Expand
Identification of Low-Abundance Lipid Droplet Proteins in Seeds and Seedlings1[OPEN]
Several previously unknown low-abundant lipid droplet proteins are identified in Arabidopsis thaliana seeds and seedlings by quantitative proteomics combined with two cell biological approaches. TheExpand
Overview of the Role of Cell Wall DUF642 Proteins in Plant Development
The regulation of pectin methyl esterases mediated by DUF642 genes could contribute to the regulation of the cell wall properties during plant growth. Expand
Transcriptomic profiling of the high-vigour maize (Zea mays L.) hybrid variety response to cold and drought stresses during seed germination
  • Heqin Li, H. Yue, +8 authors Xuwen Jiang
  • Medicine
  • Scientific reports
  • 2021
A better understanding of the molecular mechanism of response to abiotic stress during maize seed germination is provided, and could be useful for cultivar improvement and breeding of high vigour maize cultivars. Expand
Proteomics and plant biology: contributions to date and a look towards the next decade
ABSTRACT Introduction This review presents the view of the author, that is opinionable and even speculative, on the field of proteomics, its application to plant biology knowledge, and translation toExpand
Estimation of large block structured covariance matrices: Application to ‘multi‐omic’ approaches to study seed quality
This work proposes a novel, efficient and fully data-driven approach for estimating large block structured sparse covariance matrices in the case where the number of variables is much larger than thenumber of samples without limiting ourselves to block diagonal matrices. Expand
Méthodes régularisées pour l’analyse de données multivariées en grande dimension : théorie et applications.
Dans cette these nous nous interessons au modele lineaire general (modele lineaire multivarie) en grande dimension. Nous proposons un nouvel estimateur parcimonieux des coefficients de ce modele quiExpand


Mechanisms Governing the Endosomal Membrane Recruitment of the Core Retromer in Arabidopsis*
It is reported that VPS35 alone is able to bind to endosomal membranes and plays an essential role in VPS26 and VPS29 membrane recruitment and provides original information on the molecular interactions that mediate assembly of the core retromer in plants. Expand
AtVPS29, a putative component of a retromer complex, is required for the efficient sorting of seed storage proteins.
An Arabidopsis mutant, maigo 1 (mag1), which abnormally accumulates the precursors of two major storage proteins, 12S globulin and 2S albumin, in dry seeds is reported, suggesting that MAG1/AtVPS29 protein is involved in recycling a plant receptor for the efficient sorting of seed storage proteins. Expand
Analyses of SORTING NEXINs Reveal Distinct Retromer-Subcomplex Functions in Development and Protein Sorting in Arabidopsis thaliana[C][W]
It is shown by genetic and cell imaging approaches that the Arabidopsis thaliana SNX1 protein recruits SNX2 at the endosomal membrane, a process required forSNX1-SNX2 dimer activity, and SNXs are dispensable for membrane binding and function of the retromer complex. Expand
Trafficking of Vacuolar Proteins: The Crucial Role of Arabidopsis Vacuolar Protein Sorting 29 in Recycling Vacuolar Sorting Receptor[W]
It is proposed that VPS29 plays a crucial role in recycling VSRs from the PVC to the TGN during the trafficking of soluble proteins to the LV during trafficking to the lytic vacuole. Expand
Arabidopsis VPS35, a retromer component, is required for vacuolar protein sorting and involved in plant growth and leaf senescence.
The findings suggest that V PS35, mainly VPS35b, is involved in sorting proteins to PSVs in seeds, possibly by recycling VSR from PVCs to the Golgi complex, and is also involved in plant growth and senescence in vegetative organs. Expand
Vacuolar Processing Enzymes Are Essential for Proper Processing of Seed Storage Proteins in Arabidopsis thaliana*
The overall results suggest that seed-type βVPE is most essential for the processing of storage proteins, and that the vegetative-type VPEs and aspartic proteinase complementβVPE activity in this processing. Expand
Plant Vacuolar Trafficking Occurs through Distinctly Regulated Pathways
Plants have developed a complex vacuolar transport system distinct from that of nonplant systems by assigning evolutionarily conserved machinery to unique trafficking pathways, which provide a fundamental basis for plant development at the cellular and higher-ordered levels. Expand
Seed germination is blocked in Arabidopsis putative vacuolar sorting receptor (atbp80) antisense transformants.
The membrane receptor protein from pea, peabp80, has been shown to function by in vitro binding studies, and in vivo in yeast mutant, as a vacuolar sorting receptor (VSR) and, in this paper, atbp80b was over- and under-expressed in Arabidopsis. Expand
Endosome to Golgi Retrieval of the Vacuolar Protein Sorting Receptor, Vps10p, Requires the Function of the VPS29, VPS30, and VPS35 Gene Products
The data indicate that the VPS29, VPS30, and VPS35 gene products are required for the normal recycling of Vps10p from the prevacuolar endosome back to the Golgi where it can initiate additional rounds of vacuolar hydrolase sorting. Expand
Peroxisome extensions deliver the Arabidopsis SDP1 lipase to oil bodies
It is demonstrated that the core retromer, a complex involved in protein trafficking, participates in OB biogenesis, lipid store degradation, and SDP1 localization to OBs, and it is shown that the timely transfer of SDP 1 to the OB membrane requires a functional core retRomer. Expand