Combined LC/MS-platform for analysis of all major stratum corneum lipids, and the profiling of skin substitutes.

@article{Smeden2014CombinedLF,
  title={Combined LC/MS-platform for analysis of all major stratum corneum lipids, and the profiling of skin substitutes.},
  author={Jeroen van Smeden and Walter A Boiten and Thomas Hankemeier and Robert Rissmann and Joke A. Bouwstra and Rob J. Vreeken},
  journal={Biochimica et biophysica acta},
  year={2014},
  volume={1841 1},
  pages={70-9}
}
Ceramides (CERs), cholesterol, and free fatty acids (FFAs) are the main lipid classes in human stratum corneum (SC, outermost skin layer), but no studies report on the detailed analysis of these classes in a single platform. The primary aims of this study were to 1) develop an LC/MS method for (semi-)quantitative analysis of all main lipid classes present in human SC; and 2) use this method to study in detail the lipid profiles of human skin substitutes and compare them to human SC lipids. By… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 10 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 87 references

Bouwstra, LC/MS analysis of stratum corneum lipids: ceramide profiling and discovery

J. van Smeden, L. Hoppel, R. van der Heijden, T. Hankemeier, J.A.R.J. Vreeken
J. Lipid Res • 2011
View 4 Excerpts
Highly Influenced

New acylceramide in native and reconstructed epidermis.

The Journal of investigative dermatology • 2003
View 4 Excerpts
Highly Influenced

J

V. S. Thakoersing
van Smeden, A.A. Mulder, R.J. Vreeken, A. El Ghalbzouri, J.A. Bouwstra, Increased presence of monounsaturated fatty acids in the stratum corneum of human skin equivalents, J. Invest. Dermatol. 133 • 2013
View 2 Excerpts

Baillet-Guffroy, High-temperature gas chromatography– mass spectrometry for skin surface lipids

R.Michael-Jubeli, A. J. Bleton
profiling, J. Lipid Res • 2011
View 1 Excerpt

Investigating the barrier function of skin lipid models with varying compositions.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V • 2011
View 1 Excerpt

Similar Papers

Loading similar papers…