Combinatorial tetramer staining and mass cytometry analysis facilitate T-cell epitope mapping and characterization

@article{Cerundolo2013CombinatorialTS,
  title={Combinatorial tetramer staining and mass cytometry analysis facilitate T-cell epitope mapping and characterization},
  author={Vincenzo W Cerundolo and Natalia Sigal and Nitya Nair and Brian A. Kidd and Harry B Greenberg and Mark M. Davis},
  journal={Nature Biotechnology},
  year={2013},
  volume={31},
  pages={623-629}
}
It is currently not possible to predict which epitopes will be recognized by T cells in different individuals. This is a barrier to the thorough analysis and understanding of T-cell responses after vaccination or infection. Here, by combining mass cytometry with combinatorial peptide–MHC tetramer staining, we have developed a method allowing the rapid and simultaneous identification and characterization of T cells specific for many epitopes. We use this to screen up to 109 different peptide–MHC… CONTINUE READING
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The Immune Epitope Database 2.0

Nucleic Acids Research • 2010

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