Combinatorial mutagenesis of the voltage-sensing domain enables the optical resolution of action potentials firing at 60 Hz by a genetically encoded fluorescent sensor of membrane potential.

@article{Piao2015CombinatorialMO,
  title={Combinatorial mutagenesis of the voltage-sensing domain enables the optical resolution of action potentials firing at 60 Hz by a genetically encoded fluorescent sensor of membrane potential.},
  author={Hong Hua Piao and Dhanarajan Rajakumar and Bok Eum Kang and Eun Ha Kim and Bradley J. Baker},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  year={2015},
  volume={35 1},
  pages={372-85}
}
ArcLight is a genetically encoded fluorescent voltage sensor using the voltage-sensing domain of the voltage-sensing phosphatase from Ciona intestinalis that gives a large but slow-responding optical signal in response to changes in membrane potential (Jin et al., 2012). Fluorescent voltage sensors using the voltage-sensing domain from other species give faster yet weaker optical signals (Baker et al., 2012; Han et al., 2013). Sequence alignment of voltage-sensing phosphatases from different… CONTINUE READING