Combinatorial engineering of ldh-a and bcl-2 for reducing lactate production and improving cell growth in dihydrofolate reductase-deficient Chinese hamster ovary cells

@article{Jeon2011CombinatorialEO,
  title={Combinatorial engineering of ldh-a and bcl-2 for reducing lactate production and improving cell growth in dihydrofolate reductase-deficient Chinese hamster ovary cells},
  author={Min Kyoung Jeon and Da Young Yu and Gyun Min Lee},
  journal={Applied Microbiology and Biotechnology},
  year={2011},
  volume={92},
  pages={779-790}
}
In Chinese hamster ovary (CHO) cells, rapid glucose metabolism normally leads to inefficient use of glucose, most of which is converted to lactate during cell cultures. Since lactate accumulation during the culture often exerts a negative effect on cell growth and valuable product formation, several genetic engineering approaches have been developed to suppress lactate dehydrogenase-A (LDH-A), the enzyme converting pyruvate into lactate. However, despite the reduced lactate accumulation, such… CONTINUE READING

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Down-regulation of lactate dehydrogenaseA by siRNAs for reduced lactic acid formation of Chinese hamster ovary cells producing thrombopoietin

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