Combinatorial Development of Biomaterials for Clonal Growth of Human Pluripotent Stem Cells

  title={Combinatorial Development of Biomaterials for Clonal Growth of Human Pluripotent Stem Cells},
  author={Ying Mei and K. Saha and S. Bogatyrev and J. Yang and A. Hook and Z. I. Kalcioglu and S. Cho and M. Mitalipova and N. Pyzocha and Fredrick P. Rojas and K. V. Van Vliet and M. Davies and M. Alexander and R. Langer and R. Jaenisch and D. Anderson},
  journal={Nature materials},
  pages={768 - 778}
  • Ying Mei, K. Saha, +13 authors D. Anderson
  • Published 2010
  • Biology, Medicine, Materials Science
  • Nature materials
  • Both human embryonic stem (hES) cells and induced pluripotent stem (hiPS) cells can self-renew indefinitely in culture, however current methods to clonally grow them are inefficient and poorly-defined for genetic manipulation and therapeutic purposes. Here we develop the first chemically-defined, xeno-free, feeder-free synthetic substrates to support robust self-renewal of fully-dissociated hES and hiPS cells. Materials properties including wettability, surface topography, surface chemistry and… CONTINUE READING
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