Combination strategy to increase cyclosporin A productivity by Tolypocladium niveum using random mutagenesis and protoplast transformation.

@article{Lee2009CombinationST,
  title={Combination strategy to increase cyclosporin A productivity by Tolypocladium niveum using random mutagenesis and protoplast transformation.},
  author={Mi-jin Lee and Cac Thi Phung Duong and Kyuboem Han and Eung-Soo Kim},
  journal={Journal of microbiology and biotechnology},
  year={2009},
  volume={19 9},
  pages={
          869-72
        }
}
The cyclic undecapeptide cyclosporin A (CyA), one of the most valuable immunosuppressive drugs, is produced nonribosomally by a multifunctional cyclosporin synthetase enzyme complex by the filamentous fungus Tolypocladium niveum. To increase CyA productivity by wild-type T. niveum (ATCC 34921), random mutagenesis was first performed using an antifungal agar-plug colony assay (APCA) selection approach. This generated a mutant strain producing more than 9-fold greater CyA than the wild-type… 
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