Combination of overlapping bacterial artificial chromosomes by a two-step recombinogenic engineering method.

@article{Zhang2003CombinationOO,
  title={Combination of overlapping bacterial artificial chromosomes by a two-step recombinogenic engineering method.},
  author={Xin-Mei Zhang and Jian-Dong Huang},
  journal={Nucleic acids research},
  year={2003},
  volume={31 15},
  pages={e81}
}
Recombinogenic engineering or recombineering is a powerful new method to engineer DNA without the need for restriction enzymes or ligases. We report here a general method for using recombineering to combine overlapping bacterial artificial chromosomes (BACs) to build larger, unified BACs. In order to test the feasibility of using recombineering to combine two large DNA fragments (>20 kb), we constructed a unified BAC containing the full-length tyrosinase-related protein-1 (Tyrp-1) gene from two… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 1 time over the past 90 days. VIEW TWEETS
10 Extracted Citations
14 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 10 extracted citations

Referenced Papers

Publications referenced by this paper.
Showing 1-10 of 14 references

A highly ef®cient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning

  • E. C. Lee, D. Yu, +5 authors N. G. Copeland
  • BAC DNA. Genomics,
  • 2001

Similar Papers

Loading similar papers…