Combination of fluorescent in situ hybridization and microautoradiography-a new tool for structure-function analyses in microbial ecology.

@article{Lee1999CombinationOF,
  title={Combination of fluorescent in situ hybridization and microautoradiography-a new tool for structure-function analyses in microbial ecology.},
  author={Natuschka Lee and Per Halkj{\ae}r Nielsen and K H Andreasen and Stefan Juretschko and Jeppe Lund Nielsen and Karl Heinz Schleifer and Michael Wagner},
  journal={Applied and environmental microbiology},
  year={1999},
  volume={65 3},
  pages={1289-97}
}
A new microscopic method for simultaneously determining in situ the identities, activities, and specific substrate uptake profiles of individual bacterial cells within complex microbial communities was developed by combining fluorescent in situ hybridization (FISH) performed with rRNA-targeted oligonucleotide probes and microautoradiography. This method was evaluated by using defined artificial mixtures of Escherichia coli and Herpetosiphon aurantiacus under aerobic incubation conditions with… CONTINUE READING
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