Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins.

@article{Koglin2006CombinationOC,
  title={Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins.},
  author={Alexander Koglin and Christian Klammt and Nikola Trbovic and Daniel Schwarz and Birgit Schneider and Birgit Sch{\"a}fer and Frank Loehr and Frank Bernhard and Volker D{\"o}tsch},
  journal={Magnetic resonance in chemistry : MRC},
  year={2006},
  volume={44 Spec No},
  pages={S17-23}
}
Despite major technical advance in methods used for structural investigations of proteins structure determination of membrane proteins still poses a significant challenge. Recently, the application of cell-free expression systems to membrane proteins has demonstrated that this technique can be used to produce quantities sufficient for structural investigations for many different membrane proteins. In particular for NMR spectroscopy, cell-free expression provides major advantages since it allows… CONTINUE READING