Comb-type copolymer: stabilization of triplex DNA and possible application in antigene strategy.

@article{Ferdous1998CombtypeCS,
  title={Comb-type copolymer: stabilization of triplex DNA and possible application in antigene strategy.},
  author={Anwarul Ferdous and H. Watanabe and Toshihiro Akaike and Atsushi Maruyama},
  journal={Journal of pharmaceutical sciences},
  year={1998},
  volume={87 11},
  pages={
          1400-5
        }
}
By employing a reductive amination reaction between the epsilon-amino groups of poly(L-lysine) (PLL) and the reductive ends of the hydrophilic dextran (Dex) side chain, we have prepared different comb-type copolymers which varied in the degree of grafting and the length of the hydrophilic Dex chains. The resulting copolymers, poly(L-lysine)-graft-dextran (PLL-g-Dex), were tested for their ability to stabilize triplex DNA in vitro under physiologically relevant conditions. Thermal denaturation… 
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copolymer accelerates DNA hybridization by two orders.
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References

SHOWING 1-10 OF 35 REFERENCES
Characterization of interpolyelectrolyte complexes between double-stranded DNA and polylysine comb-type copolymers having hydrophilic side chains.
TLDR
By regulating the degree of grafting and the molecular weight of grafted side chains, it would be possible to design novel different graft copolymers capable of acting as carriers of functional genes to target cells or tissue.
Poly(L-lysine)-graft-dextran copolymer: amazing effects on triplex stabilization under physiological pH and ionic conditions (in vitro).
TLDR
It is suggested that the ability of the graft copolymer to stabilize triplex DNA under physiologically relevant pH and salt concentrations will be a cue for further progress in the antigene strategy.
Synthesis of novel polyampholyte comb-type copolymers consisting of a poly(L-lysine) backbone and hyaluronic acid side chains for a DNA carrier.
TLDR
These polyampholyte comb-type copolymers vary their assembling structure in water in response to two kinds of environmental factors, i.e., ionic strength and pH.
Conjugates of Oligonucleotides with Triplex-Specific Intercalating Agents. Stabilization of Triple-Helical DNA in the Promoter Region of the Gene for the α-Subunit of Interleukin 2 (IL-2Rα)
TLDR
Triplex-specific intercalators, namely benzopyridoindole and BPQ derivatives, have been conjugated to the 5‘ end or to an internucleotide position of a 15-mer oligonucleotide and found to form and stabilize a triple-helical structure involving a 42-mer duplex target.
Selectivity of polyamines in triplex DNA stabilization.
TLDR
Dinopropane stabilized the triplex DNA such that the Tm1 was 10 degrees C higher than that in the presence of an equimolar concentration of putrescine, and H2N (CH2)nNH2 was the most effective diamine to stabilize the poly(dA).2poly(dT) triplex.
Stabilization of triple helical DNA by a benzopyridoquinoxaline intercalator.
TLDR
Evidence is obtained that BPQ is actually bound to the triplex region and may distort in a sequence-specific fashion, and in vivo BPQ might enhance the formation of both undesired and desired DNA triplexes.
Positively Charged Oligonucleotides Overcome Potassium-Mediated Inhibition of Triplex DNA Formation
TLDR
It is concluded that replacement of phosphodiester bonds with positively-charged phosphoramidate linkages results in more efficient triplex formation, suggesting that these compounds may prove useful for in vivo applications.
The anti-gene strategy: control of gene expression by triplex-forming-oligonucleotides.
TLDR
The results presently available provide a rational basis for the development of new tools for cellular biology and of new therapeutical approaches to selectively control gene expression at the transcriptional level.
Triplex DNA structures.
TLDR
Intramolecular triplexes are the major elements of H-DNAs, unusual DNA structures, which are formed in homopurine-homopyrimidine regions of supercoiled DNAs.
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