Colorimetric Method for Determination of Sugars and Related Substances

@article{Dubois1956ColorimetricMF,
  title={Colorimetric Method for Determination of Sugars and Related Substances},
  author={M. Dubois and K. A. Gilles and J. Kelvin Hamilton and Paul A. Rebers and F. Smith},
  journal={Analytical Chemistry},
  year={1956},
  volume={28},
  pages={350-356}
}
Simple sugars, oligosaccharides, polysaccharides, and their derivatives, including the methyl ethers with free or potentially free reducing groups, give an orangeyellow color w-hen treated with phenol and concentrated sulfuric acid. The reaction is sensitive and the color is stable. By use of this phenol-sulfuric acid reaction, a method has been developed to determine submicro amounts of sugars and related substances. In conjunction with paper partition chromatography the method is useful for… Expand
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TLDR
Preliminary experiments showed that the anthrone and the α-naphthol sulphonate reagents give good results with pure sugar solutions, but the presence of only traces of solvents such as butanol, phenol and propionic acid used in the chromatographic separation of the sugars rendered them useless. Expand
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TLDR
The most common faults of the reagents hitherto tried are: a partial destruction of the paper, making it fragile; (2) a dark background colour; (3) poor distinction between the various classes of sugars. Expand
A Colorimetric Estimation of Sugars using Benzidine
TLDR
A quantitative technique, using this reagent, has been perfected and found to be satisfactory for the majority of common sugars and their methylated derivatives with the exception of ketoses. Expand
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The spectral characteristics of solutions of eighteen representative monosaccharides in 79 per cent sulfuric acid have been determined for solutions maintained for 2 hours at 25° and 15 minutes atExpand
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TLDR
The method using phenol–water suffers from the disadvantage that the location of the sugars on the paper by treatment with ammoniacal silver nitrate cannot be accomplished without some difficulty, and the paper quickly becomes dark or sometimes black. Expand
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TLDR
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TLDR
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THE use of ammoniacal silver nitrate solution as a spraying reagent for revealing the presence of sugars on filter-paper chromatograms1 has the advantage of general application; but it has aExpand
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TLDR
The author believed that the most suitable substance would be a compound similar to picric acid, which would be reduced by glucose to form a highly colored nitroamino compound, but dissimilar to picic acid in that it would neither form colored compounds with acetone or creatinine nor be reducing by urinary constituents other than the sugars. Expand
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DURING the course of investigations leading te the development in this laboratory of the phenol–sulphuric acid procedure for the quantitative determination of sugars1, small amounts of water-solubleExpand
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