Colorimetric Method for Determination of Sugars and Related Substances

@inproceedings{Dubois2002ColorimetricMF,
  title={Colorimetric Method for Determination of Sugars and Related Substances},
  author={Michel Y. Dubois and Arnaud Gilles and Jill K Hamilton and Paul A. Rebers and Fred Douglas Smith},
  year={2002}
}
Simple sugars, oligosaccharides, polysaccharides, and their derivatives, including the methyl ethers with free or potentially free reducing groups, give an orangeyellow color w-hen treated with phenol and concentrated sulfuric acid. The reaction is sensitive and the color is stable. By use of this phenol-sulfuric acid reaction, a method has been developed to determine submicro amounts of sugars and related substances. In conjunction with paper partition chromatography the method is useful for… CONTINUE READING
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