Colorimetric Measurement of Protein Stained with Coomassie Brilliant Blue R on Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis by Eluting with Formamide

@inproceedings{Makino1986ColorimetricMO,
  title={Colorimetric Measurement of Protein Stained with Coomassie Brilliant Blue R on Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis by Eluting with Formamide},
  author={Amane Makino and Tadahiko Mae and Koji Ohira},
  year={1986}
}
consisting in eluting Amido Black 10B with 1 N NaOH. Coomassie Bnlliant Blue R-250 (CBB-R) is widely used for staining because ef its high sensitivity. Recently, Tal et aL2} reported that CBB-R bound to protein on polyacrylamide gel (PAG) is eluted with dimethylsulfoxide. We found that CBB-R is quantitatively extracted with formamide from PAG containing sodium dodecyl sulfate (SDS) and can be measured spectrophotometrically 3) In this paper, we describe a simple method fbr this quantitatiye… CONTINUE READING

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