Colorectal Cancer-Specific Cytochrome P450 2W1: Intracellular Localization, Glycosylation, and Catalytic Activity
@article{Gomez2010ColorectalCC, title={Colorectal Cancer-Specific Cytochrome P450 2W1: Intracellular Localization, Glycosylation, and Catalytic Activity}, author={Alvin V. Gomez and Jana Nekvindov{\'a} and Sandra Travica and Mi-Young Lee and Inger Johansson and David Edler and Souren Mkrtchian and Magnus Ingelman-Sundberg}, journal={Molecular Pharmacology}, year={2010}, volume={78}, pages={1004 - 1011} }
Cytochrome P450 2W1 (CYP2W1) is expressed at high levels in colorectal cancer cells. Moreover, we have shown previously that a higher tumor expression is associated with less survival. In this study, we characterize post-translational modification, inverted endoplasmic reticulum (ER) topology, and catalytic activity of CYP2W1. The analysis of colorectal normal and cancer tissues and CYP2W1 overexpressing human embryonic kidney (HEK) 293 cells showed that a fraction of CYP2W1 is modified by N…
42 Citations
Colon Cancer Specific Cytochrome P 450 2 W 1 : Polymorphism , Membrane Topology and Endogenous Roles in Development
- Biology
- 2016
The characterized polymorphic variants of CYP2W1 are characterized and no significant correlation between their distribution and CRC risk is found and this study strongly supports the atypical membrane topology of CY P450 2W1 and suggests the interaction with redox partner yet to be identified in the ER lumen in a potential role in gut development.
The CYP2W1 enzyme: regulation, properties and activation of prodrugs
- Biology, ChemistryDrug metabolism reviews
- 2016
The CRC specific localization of CYP2W1 and its effective prodrug activation makes it a very promising target for future development of cancer therapeutics.
Expression of CYP2S1 and CYP2W1 in breast cancer epithelial cells and modulation of their expression by synthetic methoxy stilbenes.
- Biology, ChemistryPharmacological reports : PR
- 2019
Colon Cancer–Specific Cytochrome P450 2W1 Converts Duocarmycin Analogues into Potent Tumor Cytotoxins
- Biology, ChemistryClinical Cancer Research
- 2013
A novel approach for treatment of colon cancer that uses a locoregional activation of systemically inactive prodrug by the tumor-specific activator enzyme CYP2W1 is suggested.
Human Cytochrome P450 2W1 Is Not Expressed in Adrenal Cortex and Is Only Rarely Expressed in Adrenocortical Carcinomas
- Biology, MedicinePloS one
- 2016
It is concluded that normal adrenal tissue lacks P450 2W1 enzyme expression; also, adrenocortical carcinomas generally do not express the enzyme, underline the colon cancer specificity of CYP2W1 enzymes expression.
Membrane topology and search for potential redox partners of colon cancer‐specific cytochrome P450 2W1
- Biology, ChemistryFEBS letters
- 2016
The reduced catalytic activity of the Asn177 mutant that is modified by glycan moieties in the wild‐type enzyme indicates a functional relevance of CYP2W1 glycosylation.
Cytochrome P450 2W1 (CYP2W1) – ready for use as the biomarker and drug target for cancer?
- BiologyXenobiotica; the fate of foreign compounds in biological systems
- 2017
Catalytic activity of CYP2W1 should be tested on a wider spectrum of endogenous and exogenous substances before its use as the drug target of cancer because of its characteristic cancer-specific expression.
Hepatocellular carcinoma: gene expression profiling and regulation of xenobiotic-metabolizing cytochromes P450.
- Biology, MedicineBiochemical pharmacology
- 2020
The expression of CYP2W1: a prognostic marker in colon cancer.
- Biology, MedicineAnticancer research
- 2012
The results of the current study were in agreement with those of the previous pilot study and show that higher expression of CYP2W1 seems to be of prognostic value in colon cancer.
Cancer Therapy : Preclinical Colon Cancer – Speci fi c Cytochrome P 450 2 W 1 Converts Duocarmycin Analogues into Potent Tumor Cytotoxins
- Biology, Medicine
- 2013
A novel approach for treatment of colon cancer that uses a locoregional activation of systemically inactive prodrug by the tumor-specific activator enzyme CYP2W1 is suggested.
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