Colorectal Cancer-Specific Cytochrome P450 2W1: Intracellular Localization, Glycosylation, and Catalytic Activity

@article{Gomez2010ColorectalCC,
  title={Colorectal Cancer-Specific Cytochrome P450 2W1: Intracellular Localization, Glycosylation, and Catalytic Activity},
  author={Alvin V. Gomez and Jana Nekvindov{\'a} and Sandra Travica and Mi-Young Lee and Inger Johansson and David Edler and Souren Mkrtchian and Magnus Ingelman-Sundberg},
  journal={Molecular Pharmacology},
  year={2010},
  volume={78},
  pages={1004 - 1011}
}
Cytochrome P450 2W1 (CYP2W1) is expressed at high levels in colorectal cancer cells. Moreover, we have shown previously that a higher tumor expression is associated with less survival. In this study, we characterize post-translational modification, inverted endoplasmic reticulum (ER) topology, and catalytic activity of CYP2W1. The analysis of colorectal normal and cancer tissues and CYP2W1 overexpressing human embryonic kidney (HEK) 293 cells showed that a fraction of CYP2W1 is modified by N… 
Colon Cancer Specific Cytochrome P 450 2 W 1 : Polymorphism , Membrane Topology and Endogenous Roles in Development
Cytochrome P450 2W1 (CYP2W1) belongs to a family of drug metabolizing monooxygenases with an unidentified yet endogenous function. CYP2W1 expression pattern has characteristic features of oncofetal
The CYP2W1 enzyme: regulation, properties and activation of prodrugs
TLDR
The CRC specific localization of CYP2W1 and its effective prodrug activation makes it a very promising target for future development of cancer therapeutics.
Expression of CYP2S1 and CYP2W1 in breast cancer epithelial cells and modulation of their expression by synthetic methoxy stilbenes.
TLDR
Stilbenes can modulate the expression of "orphan" CYPs more efficiently than resveratrol, although their relatively low level of expression suggests that they may be less involved in the transformation of therapeutic agents in these types of tumors.
Colon Cancer–Specific Cytochrome P450 2W1 Converts Duocarmycin Analogues into Potent Tumor Cytotoxins
TLDR
A novel approach for treatment of colon cancer that uses a locoregional activation of systemically inactive prodrug by the tumor-specific activator enzyme CYP2W1 is suggested.
Human Cytochrome P450 2W1 Is Not Expressed in Adrenal Cortex and Is Only Rarely Expressed in Adrenocortical Carcinomas
TLDR
It is concluded that normal adrenal tissue lacks P450 2W1 enzyme expression; also, adrenocortical carcinomas generally do not express the enzyme, underline the colon cancer specificity of CYP2W1 enzymes expression.
Membrane topology and search for potential redox partners of colon cancer‐specific cytochrome P450 2W1
TLDR
The reduced catalytic activity of the Asn177 mutant that is modified by glycan moieties in the wild‐type enzyme indicates a functional relevance of CYP2W1 glycosylation.
Cytochrome P450 2W1 (CYP2W1) – ready for use as the biomarker and drug target for cancer?
  • Yan Pan, E. Ong
  • Biology, Medicine
    Xenobiotica; the fate of foreign compounds in biological systems
  • 2017
TLDR
Catalytic activity of CYP2W1 should be tested on a wider spectrum of endogenous and exogenous substances before its use as the drug target of cancer because of its characteristic cancer-specific expression.
Hepatocellular carcinoma: gene expression profiling and regulation of xenobiotic-metabolizing cytochromes P450.
TLDR
A significant down-regulation in European Caucasian patients of cytochromes P450 (CYPs), the major xenobiotic-metabolizing enzymes, in HCC tumour samples as compared to their surrounding non-cancerous (reference) tissue is demonstrated.
The expression of CYP2W1: a prognostic marker in colon cancer.
TLDR
The results of the current study were in agreement with those of the previous pilot study and show that higher expression of CYP2W1 seems to be of prognostic value in colon cancer.
Cancer Therapy : Preclinical Colon Cancer – Speci fi c Cytochrome P 450 2 W 1 Converts Duocarmycin Analogues into Potent Tumor Cytotoxins
Purpose: Cytochrome P450 2W1 (CYP2W1) is a monooxygenase detected in 30% of colon cancers, whereas its expression in nontransformed adult tissues is absent, rendering it a tumor-specific drug target
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