• Biology, Medicine
  • Published in Stem cells 1982

Colony stimulating factor in human mixed leukocyte cultures.

  title={Colony stimulating factor in human mixed leukocyte cultures.},
  author={Akira Horikoshi and Yumiko Iizuka and Umihiko Sawada and Takeshi Oshima and Ichita Amaki and Jeffrey L. Rossio and Martin John Murphy},
  journal={Stem cells},
  volume={2 4},
Colony stimulating factor (CSF) is important for granulocyte/macrophage proliferation and differentiation. We investigated the relationship between the immune reactivity and the production of CSF by using two-way mixed leukocyte cultures (MLC) prepared from human peripheral mononuclear cells obtained with the aid of a blood cell separator. Assessed over 9 days of MLC incubation, the level of CSF in the supernatant reached a maximum at day 7 and then decreased. Optimal number of MLC cells ranged… CONTINUE READING

Topics from this paper.