• Biology, Medicine
  • Published in Stem cells 1982

Colony stimulating factor in human mixed leukocyte cultures.

@article{Horikoshi1982ColonySF,
  title={Colony stimulating factor in human mixed leukocyte cultures.},
  author={Akira Horikoshi and Yumiko Iizuka and Umihiko Sawada and Takeshi Oshima and Ichita Amaki and Jeffrey L. Rossio and Martin John Murphy},
  journal={Stem cells},
  year={1982},
  volume={2 4},
  pages={
          203-17
        }
}
Colony stimulating factor (CSF) is important for granulocyte/macrophage proliferation and differentiation. We investigated the relationship between the immune reactivity and the production of CSF by using two-way mixed leukocyte cultures (MLC) prepared from human peripheral mononuclear cells obtained with the aid of a blood cell separator. Assessed over 9 days of MLC incubation, the level of CSF in the supernatant reached a maximum at day 7 and then decreased. Optimal number of MLC cells ranged… CONTINUE READING

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