Colony formation of Blastocystis hominis in soft agar

  title={Colony formation of Blastocystis hominis in soft agar},
  author={S. W. Tan and M. Singh and E. H. Yap and L. C. Ho and K. T. Moe and Josephine Howe and G. C. Ng},
  journal={Parasitology Research},
Abstract This is the first description of a method for growing axenized Blastocystis hominis as colonies in petri dishes containing soft agar. Blastocystis cells cultured in two types of agar appeared to show different colonial morphologies as well as differing colony yields. Microscopic examination of the colonies revealed many amoeboid and giant cells. Many cells were also shown to possess thin filament-like structures that appeared to stretch across the central vacuole. 
Clonal growth of Blastocystis hominis in soft agar with sodium thioglycollate
Five human Blastocystis isolates chosen for this study showed no apparent variation in colonial morphology, and isolated colonies were also successfully grown in liquid medium, providing a means of obtaining large numbers of B. hominis cells that had arisen from a single clone. Expand
Colony growth as a step towards axenization of Blastocystis isolates
  • G. Ng, K. Tan
  • Biology, Medicine
  • Parasitology Research
  • 1999
Abstract Colonies of Blastocystis from non-axenic cultures grown in agar medium were isolated from bacterial colonies. Axenization of human and reptilian isolates of Blastocystis was achieved usingExpand
Colony growth of Philippine isolates of Blastocystis hominis in simplified, soft agar medium
This is the first report on agar cloning of Blastocystis in a compound medium and analysis of the SSU rDNA genetically established the identity of the clones to be B. hominis. Expand
Morphology and reproductive mode of Blastocystis hominis in diarrhea and in vitro
The in vitro culture of Blastocystis hominis in Roswell Park Memorial Institute (RPMI) 1640 medium containing 20% calf serum is described and three distinct morphological forms, vacuolar, granular and amoeboid, were distinguished in faeces and in vitro cultures. Expand
Description of a Blastocystis species from Rattus norvegicus
Two isolates (WR1 and WR2) of Blastocystis from laboratory-bred Wistar rats were axenized by a method of colony growth in soft agar combined with antibiotic treatment, resulting in the first report of cyst formation in in vitro culture. Expand
Encystation—survival of Blastocystis hominis in immunocompetent mice abdomen cavity
Results showed that encystation enable the parasite to avoid the immune attack in competent host and simultaneously decrease the pathogenicity to host. Expand
Recent advances in Blastocystis hominis research: hot spots in terra incognita.
This review describes and discusses recent advances in B. hominis research, with particular focus on new, and sometimes controversial, information that has shed light on its genetic heterogeneity, taxonomic links, mode of transmission, in vitro culture and pathogenesis. Expand
Recent advances in Blastocystis sp . research
lastocystis sp. is a eukaryotic protozoan symbiont commonly found in the gut of humans and animals. Its role in the gut remains controversial due to conflicting reports of its pathogenicity. ThisExpand
Methods for Cultivation of Luminal Parasitic Protists of Clinical Importance
This review discusses the methods and media that are most widely used for the establishment and maintenance of the following organisms in culture: Entamoeba histolytica, Giardia intestinalis, Trichomonas vaginalis, DientamoebA fragilis, Blastocystis hominis, and Balantidium coli. Expand
Survival of Blastocystis hominis clones after exposure to a cytotoxic monoclonal antibody.
It is suggested that a heterogenous population exists in continuously maintained cultures of B. hominis and that not all cells within a certain population would be susceptible to the cytotoxic effects of 1D5. Expand


Clonal growth of Entamoeba histolytica and other species of Entamoeba in agar.
It was demonstrated in a study with the drug metronidazole that the agar colony method was useful for quantifying cell viability in drug testing, and was dependent upon filling deep vessels with agar to maintain reduced oxygen tension. Expand
A Simple, Highly Efficient Plating Method for Trypanosomatids1
A simple technique for plating trypanosomatids includes use of plates with lower agar concentrations than those usually prescribed for plating bacteria and a simple system to prevent dehydration dueExpand
Clonal growth in Petri dishes of Entamoeba histolytica.
  • D. E. Mueller, W. Petri,
  • Biology, Medicine
  • Transactions of the Royal Society of Tropical Medicine and Hygiene
  • 1995
The cultivation and cloning of Leishmania
  • 1984
Colonial morphology of Trichomonas vaginalis in Agar.