Colocalization of fluorescent probes: accurate and precise registration with nanometer resolution.

@article{Churchman2012ColocalizationOF,
  title={Colocalization of fluorescent probes: accurate and precise registration with nanometer resolution.},
  author={Lee Stirling Churchman and James A Spudich},
  journal={Cold Spring Harbor protocols},
  year={2012},
  volume={2012 2},
  pages={
          141-9
        }
}
Colocalization of fluorescent probes is commonly used in cell biology to discern the proximity of two proteins in the cell. Considering that the resolution limit of optical microscopy is on the order of 250 nm, there has not been a need for high-resolution colocalization techniques. However, with the advent of higher resolution techniques for cell biology and single-molecule biophysics, colocalization must also improve. For diffraction-limited applications, a geometric transformation (i.e… CONTINUE READING

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