Collection of embryos from Darwin's finches (Thraupidae, Passeriformes).

  title={Collection of embryos from Darwin's finches (Thraupidae, Passeriformes).},
  author={Arhat Abzhanov},
  journal={Cold Spring Harbor protocols},
  volume={2009 3},
There are no breeding colonies of Darwin’s finches anywhere in the world. Thus, all of the embryonic material is collected in the wild. This protocol describes how, in a field setting, fertilized eggs are collected and incubated at a precise temperature and how the resulting embryos are harvested and processed for in situ hybridization, antibody staining, and microarray analyses. In addition, the protocol includes steps for preparing the heads of older embryos for histological staining of bone… CONTINUE READING

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