Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations

  title={Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations},
  author={Birger Neuhaus and Thomas Schmid and Jens Riedel},
A wide range of aspects concerning microscope slides, their preparation, long-time storage, curatorial measures in collections, deterioration, restoration, and study is summarized based on our own data and by analyzing more than 600 references from the 19 th century until 2016, 15 patents, and about 100 Materials Safety Data Sheets. Information from systematic zoology, conservation sciences, chemistry, forensic sciences, pathology, paleopathology, applied sciences like food industry, and most… Expand
Ageing Effects in Mounting Media of Microscope Slide Samples from Natural History Collections: A Case Study with Canada Balsam and PermountTM
The results question whether or not Canada balsam should really be regarded as a mounting medium that lasts for centuries, if its increasing degree of polymerisation can lead to a mount which is no longer restorable. Expand
VIRMISCO – The Virtual Microscope Slide Collection
The Virtual Microscope Slide Collection – VIRMISCO project has developed recommendations for taking microscopic image stacks of three-dimensional objects, depositing and presenting such series of digital image files or z-stacks as an online platform. Expand
A Guide to Preparation Protocols in Palynology
ABSTRACT A comprehensive, illustrated guide to the preparation (i.e. extraction, concentration and microscope slide production) of palynomorphs from samples of sediments, sedimentary rocks and otherExpand
Rehydration of dried mushroom specimens with Aerosol® OT for scanning electron microscopy
Morphological, anatomical and ultrastructural characteristics are important for taxonomical and phylogenetic studies of fungi. For scanning electron microscopy (SEM), usually only dry voucherExpand
Clean and Constrain: a pest management protocol and an overview of some collections management considerations for microscope slide collections
An unexpected infestation in microscope slide cabinets is described and a protocol for controlling pests in collections that cannot be frozen is outlined, while touching on additional issues relating to the use and management of microscope slide collections. Expand
Optimal Preparation of Tissue Sections for Light-Microscopic Analysis of Phloem Anatomy.
  • M. R. Pace
  • Medicine, Chemistry
  • Methods in molecular biology
  • 2019
The goal of this chapter is to present a detailed protocol containing various techniques for optimal preparation of phloem tissue samples for light microscopic analysis. Expand
Airyscan superresolution microscopy: A high-throughput alternative to electron microscopy for the visualization and analysis of fossil pollen
Abstract Airyscan confocal superresolution microscopy is a new optical technique that can detect morphological features smaller than the diffraction limit of light. It captures both the external andExpand
A further modification of Dioni’s mounting media to allow staining, clearing and mounting of Acari
Modified Dioni’s media has previously been presented as an alternative to tradition Gum Chloral mounting media for microscopical specimens. This paper aims to explore a further modification ofExpand
ICZN Declaration 45: a remedy for the nomenclatural and typification dilemma regarding soft-bodied meiofaunal organisms?
Although ICZN Declaration 45 already shed some light to the issue regarding “typeless species descriptions,” we here explore the assignment of new species-group taxa in the absence of a physicalExpand
Basic Histological Techniques for Planarians.
The chapter provides a concise account of collecting techniques, and basic histological techniques for investigating planarians, intended for use by practicing scientists and postgraduate studentsExpand


A Review of Techniques Used in the Preparation, Curation and Conservation of Microscope Slides at the Natural History Museum, London
Permanent microscope slide mounts have been an integral part of the Natural History Museum's (NHM) collections since microscopes were first used in the study of natural history specimens. This paperExpand
Raman spectroscopy as a tool for the collection management of microscope slides
In this contribution, a thorough study of the applicability of Raman spectroscopy for the described task is presented, demonstrating the successful feasibility of the chosen method for a clear distinction between different media and an unambiguous identification of real unknown samples by a distinct assignment to a previously recorded spectral library. Expand
The preparation and preservation of collections
Subjects covered include: The history of preparation and preservation of natural history collections, preservation policy, and histochemical tests for nucleic acids, proteins, fat and oil, and carbohydrates. Expand
Observations on the curation of plant and soil nematodes
Observations are made on the curation of plant and soil nematodes by reference to the extensive collection of microscope slides and specimens in the Nematology Department, Rothamsted ExperimentalExpand
An improved clearing and mounting solution to replace chloral hydrate in microscopic applications1
Visikol is as effective as chloral hydrate in providing clarity and resolution of all tissues examined, allowing observation of deeper layers of cells and making it effective in research, botanical and quality control, and for educational applications. Expand
Mounting media: An overview
An attempt is made to review, venture and summarize on various types of mounting media and their uses in routine histopathologically and immunochemical staining. Expand
On Mounting Delicate Bryophytes in Glycerol
Glycerine jelly is an effective substitute microscopic mounting medium for now difficult-to-obtain gum chloral preparations and has been superseded in bryological work to a great extent by Hoyer's Solution, which is more convenient to work with since it does not need to be heated. Expand
1.6.3 Preparation, mounting and descriptive study of eriophyoid mites
This chapter reviews methods for preparing and preserving eriophyoid mites, and recommends a substitute for gum arabic in media—benzophenone-tetracarboxylic dianhydride (BTDA)—which resulted in more permanent slides. Expand
Recommended procedures and techniques for morphological studies of Eriophyoidea (Acari: Prostigmata)
Based on their expertise on this topic, the authors provide guidelines and advices of the main critical aspects in managing eriophyoid mites in order to supplement and improve techniques for handling and preparation of specimens, and for improving their taxonomic study. Expand
Optimizing specimen processing for ancient soft tissue specimens
It was found that in desiccated samples, tissue architecture was optimized by using Ruffer's solution for rehydration and Schaffer's solution as fixative, because these tissues require water restoration within the tissues due to their compacted character. Expand