Collapse temperature of solutions important for lyopreservation of living cells at ambient temperature

  title={Collapse temperature of solutions important for lyopreservation of living cells at ambient temperature},
  author={Geer Yang and Kyle Gilstrap and Aili Zhang and Lisa Xuemin Xu and Xiaoming He},
  journal={Biotechnology and Bioengineering},
In this study, the collapse temperature was determined using the freeze‐drying microscopy (FDM) method for a variety of cell culture medium‐based solutions (with 0.05–0.8 M trehalose) that are important for long‐term stabilization of living cells in the dry state at ambient temperature (lyopreservation) by freeze‐drying. Being consistent with what has been reported in the literature, the collapse temperature of binary water‐trehalose solutions was found to be similar to the glass transition… 

Acquisition of collapse temperature and the influencing factors during freeze-drying of placental decellularized matrix in freeze-drying microscopy based on image processing techniques

Abstract The placenta-derived extracellular matrix is an ideal material for making freeze-dried sponges as a wound dressing. In order to prepare a porous sponge, collapse temperature is one of the

The Effect of Human Serum Albumin and Hematocrit on the Cake Collapse Temperature of Lyophilized Red Blood Cells.

Freeze-drying, or lyophilization, has shown great promise in addressing many of the logistical challenges of storing and preserving red blood cells (RBCs). A crucial part of any RBC lyophilization

Thermostability of Biological Systems: Fundamentals, Challenges, and Quantification

  • X. He
  • Engineering, Biology
    The open biomedical engineering journal
  • 2011
This review examines the fundamentals and challenges in engineering/understanding the thermostability of biological systems over a wide temperature range (from the cryogenic to hyperthermic regimen) to understand the mechanism of thermal injury and its quantification at the molecular, cellular and tissue/organ levels.

Freeze drying microscopy as a tool to study sublimation kinetics

Freeze-drying is the process of removal of water or organic solvent from a desired product by means of sublimation at a low temperature and low pressure. It is commonly employed for drying samples

Water Vapor Pathways during Freeze-Drying of Foamed Product Matrices Stabilized by Maltodextrin at Different Concentrations

This study aimed to identify the water-vapor transport mechanisms through an aerated matrix during microwave freeze-drying. Due to the larger surface area and lower water vapor transport resistance

Impact of matrix properties on the survival of freeze-dried bacteria.

Polymer-based formulations can support cell survival as effectively as disaccharides if formulation properties of importance for maintaining cell viability are identified and controlled.



Freeze-Dry Microscopy: Impact of Nucleation Temperature and Excipient Concentration on Collapse Temperature Data

Tn was found to be non-correlated with concentrations and only weakly correlated with Toc, and Toc correlated much better with the corresponding concentrations, resulting in a quadratic fit for PVP and a linear fit for 2-(hydroxypropyl)-ß-cyclodextrin.

Thermophysical Properties of Trehalose and Its Concentrated Aqueous Solutions

The authors' solubility measurements, which have allowed long times for attainment of chemical equilibrium, are substantially different from those reported earlier that used different techniques.

Physical chemistry of freeze-drying: measurement of sublimation rates for frozen aqueous solutions by a microbalance technique.

The temperature dependence of Rp suggests that, as the sample temperature approaches the eutectic temperature, hydrodynamic surface flow of adsorbed water is an important flow mechanism.

Effect of trehalose on bioluminescence and viability of freeze-dried bacterial cells

Two recombinant bacteria containing luxAB showed an increased tolerance to stresses associated with lyophilization, when the cells were freeze-dried in the presence of Trehalose, and it was tentatively concluded that trehalose played a role as a protective agent in the freeze-Drying of bacterial cells.

Literature review: supplemented phase diagram of the trehalose-water binary mixture.

The literature data were found to be reasonably consistent, with substantial agreement on the melting points for water, trehalose, andtrehalose dihydrate and the glass transition temperature of water and the solubility, freezing, and glass transition curves.

The ice nucleation temperature determines the primary drying rate of lyophilization for samples frozen on a temperature-controlled shelf.

The results document that factors such as particulate content and vial condition, which influence ice nucleation temperature, must be carefully controlled to avoid, for example, lot-to-lot variability during cGMP production.

Beneficial effect of intracellular trehalose on the membrane integrity of dried mammalian cells.

The results show that a key component of mammalian cells can be preserved in a dried state for weeks under mild conditions and thereby suggest new approaches to preserving mammalian cells.

Stability of dry liposomes in sugar glasses.

Desiccation Kinetics and Biothermodynamics of Glass Forming Trehalose Solutions in Thin Films

In this study, the desiccation kinetics of aqueous trehalose solutions were investigated numerically by solving the coupled heat and mass transfer problem with a moving interface using the finite element method and it was found that the temperature in the film could drop significantly upon the initiation of drying.