Collagenolytic activities with differentiation of the cultured cells from the murine hair apparatus.

@article{Obana1996CollagenolyticAW,
  title={Collagenolytic activities with differentiation of the cultured cells from the murine hair apparatus.},
  author={N Obana and Mitsuyasu Ito and Takashi Kobayashi and Yoshio Nagai},
  journal={Journal of dermatological science},
  year={1996},
  volume={13 1},
  pages={83-6}
}
Immature hair apparatus cells were cultured for 3 days to allow to proliferate and differentiate into several subpopulations including hair-shaft type cells on day 6. Zymographic and immunocytochemical studies of the culture media were performed to examine whether the differentiated and/or immature cells from the murine hair apparatus can secrete type IV collagenases. Zymography showed the presence of 72 and 92 kDa type IV collagenases in media, in which hair apparatus cells had been cultured… CONTINUE READING

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