Collagenase as a useful tool for the analysis of plant cellular peripheries.

@article{Luczak2015CollagenaseAA,
  title={Collagenase as a useful tool for the analysis of plant cellular peripheries.},
  author={Magdalena Luczak and Weronika Krzeszowiec-Jeleń and Dorota Konopka-Postupolska and Przemysław Wojtaszek},
  journal={Phytochemistry},
  year={2015},
  volume={112},
  pages={195-209}
}
A technique for the selective loosening of the cell wall structure and the isolation of proteins permanently knotted in the cell walls was elaborated. Following treatment with collagenase, some proteins, such as calreticulin (CRT) and auxin binding protein 1 (ABP1) were released from purified cell walls, most probably through destruction of respective interacting proteins. The results were confirmed by the immunolocalization of the ABP1 and CRT with confocal and electron microscopy. On the… CONTINUE READING
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